exosomes, the researchers built a device using two such microfluidic units in series. The first uses sound waves to remove cells and platelets from a blood sample, while the second uses sound waves of a higher frequency to separate exosomes from slightly larger extracellular vesicles.
According to the researchers, it takes less than 25 minutes to process a 100-microliter undiluted blood sample using their device.
“The new technique can address the drawbacks of the current technologies for exosome isolation, such as long turnaround time, inconsistency, low yield, contamination, and uncertain exosome integrity,” says Tony Jun Huang, a professor of mechanical engineering and materials science at Duke University. “We want to make extracting high-quality exosomes as simple as pushing a button and getting the desired samples within 10 minutes.”
"The capability of this method to separate these nanoscale vesicles, essentially without altering their biological or physical characteristics, offers appealing possibilities for developing new ways of assessing human health as well as the onset and progression of diseases," adds Subra Suresh, president-designate of Nanyang Technological University in Singapore, MIT’s Vannevar Bush Professor of Engineering Emeritus, and a former dean of engineering at MIT.
The researchers now plans to use this technology to seek biomarkers that can reveal disease states. For more, see " Isolation of exosomes from whole blood by integrating acoustics and microfluidics ."
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